Lutzomyia longipalpis Saliva Triggers Lipid Body Formation and Prostaglandin E2 Production in Murine Macrophages
Figure 5
COX-2 and PGE-synthase co-localize within lipid bodies induced by L. longipalpis SGS.
Peritoneal macrophages were stimulated with SGS (1.5 pair/well) for 24 hours. BODIPY probe-labeled lipid bodies were visualized as green punctuate intra-cytoplasmic inclusions (A and D). COX-2 (B) and PGE-synthase (E) were localized with anti-COX-2 and anti- PGE-synthase antibodies, respectively. Merged images show co-localization of COX-2 (C) and PGE-synthase (F) within lipid bodies.