Lutzomyia longipalpis Saliva Triggers Lipid Body Formation and Prostaglandin E2 Production in Murine Macrophages
Figure 3
Lipid body formation induced by SGS in vivo.
C57BL/6 mice were injected i.p. with saline or SGS (0.5 pair/cavity). One, 3, 6 and 24 hours after stimulation, cells were harvested from the peritoneal cavity and stained with the neutral lipid probe BODIPY 493/503. Kinetics of LB formation in mononuclear (A) and polymorphonuclear (B) cells. Mean fluorescence intensity (MFI) histograms of mononuclear (C) and polymorphonuclear (D) cell populations at the 3-hour time point. Dotted lines indicate unstained cells, full lines indicate stained cells from the saline group (empty curves) and from the SGS-treated group (filled curves). LBs in mononuclear cells stimulated with saline (E) or SGS (F) for 3 h detected by fluorescence microscopy, nuclei stained with DAPI. Groups were compared using Student's t test at each time point. *, p<0.05. MO, mononuclear; PMN, polymorphonuclear.