Functional Impairment of Human Myeloid Dendritic Cells during Schistosoma haematobium Infection
Figure 1
Reduced frequencies of mDC and pDC in blood from S. haematobium-infected subjects.
(A) Blood DC were identified in fixed PBMC as HLA-DR+/CD14−/CD19− cells and subsequently subdivided into mDC and pDC on the basis of positive staining for BDCA-1 and BDCA-2, respectively. Data from one representative donor is shown. (B) Frequencies of blood DC subsets in total PBMC (C) and their surface expression of HLADR, CD80, CD86 and CCR7 was determined by following the gating strategy shown in (A). (B) Box and whiskers with 10–90% percentile are shown. (C) Bars represent mean + SD. (B+C) Each group represents data from 20 donors.