Molecular Evidence for a Functional Ecdysone Signaling System in Brugia malayi
Figure 3
Bma-EcR expression in adult females, males and microfilaria.
Northern blot analysis of total RNA from B. malayi females (F), L1 larvae (L1) and males (M) shows the presence of a predominant RNA species of approximately 3.5–4 kb (marked by an asterisk). The ethidium bromide-stained ribosomal RNA (below) reflects the amount of total RNA loaded in each lane. The apparent difference in mobility of the L1 sample is probably associated with electrophoresis rather than actual mRNA size, as it is also observed in both ribosomal RNA species detected by ethidium bromide (lower panel).