Diversity of Micrurus Snake Species Related to Their Venom Toxic Effects and the Prospective of Antivenom Neutralization
Figure 4
Determination of the hyaluronidase activity.
Samples of Micrurus venoms (30 µg), or a mixture of M. frontalis (15 µg) and M. corallinus (15 µg), were incubated for 15 min at 37°C with the hyaluronic acid as substrate. After this period, it was added cetyltrimethylammonium bromide, to develop the turbidity in the mixtures, and the absorbance measured in a spectrophotometer at λem 405 nm. Results are representative for three separate experiments and expressed in units of turbidity reduction (UTR) per mg of venom.