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Field Validation of a Transcriptional Assay for the Prediction of Age of Uncaged Aedes aegypti Mosquitoes in Northern Australia

Figure 3

Assessments of the capacity of transcriptional age grading to differentiate two mosquito populations with differing mean life expectancies.

A. Experimental age predictions showing interpolated mean (solid line) and standard deviations (broken lines) that were used to generate cumulative normal distributions for each age. Ages were then randomly selected from these distributions and assigned to each individual sampled from a population defined by a given ex value. Random samples of 100, 200, 300, 400 and 500 female mosquitoes were modeled. Symbols indicate mean ex values for 999 replicates and bars show 95% confidence limits. B. Estimated population ex values generated from random samples of different sizes when ages were predicted without error. C. Estimated population ex values from different sample sizes when mosquito ages were predicted from the experimental error distributions for age predictions achieved in this study. Symbols indicate mean ex values and bars show 95% confidence limits.

Figure 3

doi: https://doi.org/10.1371/journal.pntd.0000608.g003