Role of the Endogenous Antioxidant System in the Protection of Schistosoma mansoni Primary Sporocysts against Exogenous Oxidative Stress
Figure 5
In vitro cell-mediated cytotoxicity (CMC) assay results.
Schistosoma mansoni sporocysts, cultured for 7 days in medium containing antioxidant (GPx, Prx1/2, GST26 and 28, SOD) or control GFP dsRNA were incubated for 24 hr with plasma-free hemocytes from the susceptible NMRI snail strain of Biomphalaria glabrata followed by assessment of larval mortality by propidium iodide staining. Co-culture of GST26, GST28 and Prx1/2 dsRNA-treated sporocysts with snail hemocytes resulted in small, but significant increases in percent larval mortality when compared to GFP dsRNA controls. The GPx dsRNA-treatment exhibited a nonsignificant increase in larval killing, while mortality of SOD dsRNA-treated sporocysts showed no difference compared to GFP dsRNA controls. *P≤0.04; N = 4.