IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
Fig 3
Change of cell phenotype in the lungs of mice after resuscitated hemorrhagic shock and tissue trauma (HS/T) and the cell resource of type 2 cytokine interleukin (IL) 5.
A: IL5+ gate from lung CD45+ cells was increased in the lung after HS/T, peaked at 6 hours after HS/T (n = 5–9/group). B: The percentage of Neutrophils (Ly6G+CD11b+ gated from CD45+ cells) in the lungs was increased after HS/T and peaked at 6 hours, while the frequency of T cells (CD45+CD3e+), B cells (CD45+CD45R+), and natural killer (NK) cells (CD45+NK1.1+) gradually decreased in the lung after HS/T (n = 5–8/group). C: IL5 in the lung after HS/T was observed mostly in the neutrophils (Ly6G+CD11b+). D: IL5+ neutrophils in the lung had higher CXCR2+ expression compared with the IL5- population (see histogram: the right peak shows the IL5+ neutrophils and the left peak shows the IL5- subset). * P < 0.05. FSC-H, forward scatter-height.