Effects of ADMA upon Gene Expression: An Insight into the Pathophysiological Significance of Raised Plasma ADMA
Figure 4
ADMA Alters PRMT3 Gene Expression and Protein Levels
(A) PRMT3 levels are changed by ADMA on U133A GeneChips where n = 3 (untreated versus 100 μM: 1.88-fold increase, p = 0.034).
(B) PRMT3 mRNA is changed in HCAEC following 24-h exposure to ADMA (2 and 100 μM) but not SDMA (100 μM) or L-NIO (100 μM), measured by Q-PCR (*p < 0.05 and **p < 0.01, where n = 8).
(C) PRMT3 protein levels are increased in HCAEC following 24-h treatment with ADMA (2 and 100 μM) as determined by Western blotting, using a commercially available PRMT3 antibody (Upstate Biotechnology), where equal amounts of protein were loaded in each lane. Densitometry of the PRMT3 was carried for each of the blots from four separate experiments. ADMA (2 and 100 μM) treatment for 24 h significantly increased the levels of PRMT3 (*p < 0.05). The inset blot is a representative from four separate experiments.