The ANC-1 (Nesprin-1/2) organelle-anchoring protein functions through mitochondria to polarize axon growth in response to SLT-1
Fig 3
ANC-1A and ANC-1C are enriched in touch receptor neurons and are localized to the base of the proximal axon.
(A) Expression of ANC-1ΔCH-GFP, a marker for ANC-1A and ANC-1C, in the ALM neuron. (B) Expression of ANC-1ΔCH-GFP in the PLM neuron. Also see S1 Fig for colocalization with the Touch Receptor Neuron marker. (C) Expression of Scarlet::ANC-1A in the ALM (red circle) and AVM (yellow circle). Expression also seen in the seam cells (see arrow). (D) Expression of Scarlet::ANC-1A in the PLM (blue circle). (E) Expression of Scarlet::ANC-1C in the ALM (red circle) and the AVM (yellow circle). (F) Expression of Scarlet::ANC-1C in the PLM (blue circle). (G) ANC-1 visualized by split GFP. The ANC-1 protein was endogenously tagged with 2XGFP11 and GFP1-10 was expressed by a transgene (Pmec-4::gfp1-10). (H) Schematic of mutant forms of ANC-1. Anterior is to the left. Scales bars are 5 μm.