Yeast-based evolutionary modeling of androgen receptor mutations and natural selection
Fig 1
Correlation of AR-mediated reporter activities between designer yeast and mammalian systems.
(A) Schematic representation of our designer yeast platform compared with the mammalian luciferase reporter system. (B) The list of 56 PCa-associated AR-LBD mutations accumulated in ARDB during a 22-year period (from 1990 to 2012). Top ten frequent mutations were marked in blue. (C), The Correlation analyses were based on fold changes in activation of AR-LBD mutants as normalized to the wild-type AR between the yeast and mammalian reporter systems, using the top ten frequent mutations in (B) against a variety of concentrations of DHT, E2, PROG, and CPA. Pearson’s correlation coefficient (R) and P-values were listed in the figures. Hormone concentrations in the yeast assay were DHT 10−8 M, E2 10−5 M, PROG 10−5 M, and CPA 10−5 M. Hormone concentrations in the human Hep3B reporter assay were DHT 10−9 M, E2 10−7 M, PROG 10−9 M, and CPA 10−7 M.