Cell competition is driven by Xrp1-mediated phosphorylation of eukaryotic initiation factor 2α
Fig 1
ER stress causes cell competition.
(A and B) Adult eye bearing eyFLP-induced mosaics of wild-type (A) or wolccp-28-/- (B) clones. (C) Adult eye bearing wolccp-28-/- clones with surrounding wild-type tissue removed by GMR-hid and cell-lethal mutations. (D-G) Eye disc bearing eyFLP-induced MARCM clones of wild-type (D), wolccp-28-/- (E), wolccp-28-/- + UAS-p35 (F) cells or wolccp-28-/- + UAS-wol (G) cells. (H) Quantification of the relative size of GFP-labeled clones shown in D-G. Error bars, SD; ***p<0.001 by Steel-Dwass test. (I-J’) Wing disc bearing UbxFLP-induced MARCM clones of wild-type (I) or wolccp-28-/- (J) stained with anti-cleaved Dcp-1. A magnified image of the inset in J is shown in J’. (K) Quantification of the number of dying cells at the boundary between wild-type and wolccp-28-/- clones in the wing discs. Error bars, SD; ***p<0.001 by Welch’s t-test. (L and M) Eye disc bearing eyFLP-induced MARCM clones of UAS-Xbp1-GFP (L) or wolccp-28-/- + UAS-Xbp1-GFP (M) cells stained with anti-GFP. Scale bars, 50μm. See S1 Text for detailed genotypes.