Split versions of Cleave and Rescue selfish genetic elements for measured self limiting gene drive
Fig 3
Population dynamics of Killer-Rescue/Cargo, ClvR and split50cM ClvR for elements with different fitness costs, and introduction percentages.
Split50cM ClvR provides strong drive, with Rescue/Cargo/gRNAs spending prolonged time at high frequency even in the presence of significant fitness costs. (A-F) Population dynamics modeling of different drives introduced at a 50% release percentage, with fitness cost per allele varying from 0–15%. Fitness costs per transgene allele are 0% (purple), 2.5% (blue), 5% (orange), 7.5% (yellow), 10% (green), and 15% (red). Genotype frequencies of Rescue/Cargo and Killer or Cas9 are shown. Genotype frequencies are also shown for ClvR. Allele frequencies are shown for LOF. These are indicated with solid lines following the initial introduction. Genotype and allele frequencies following a 50% WT introductions at generation 150 are indicated in dotted lines. (G-I). Heat maps showing the average Rescue/Cargo genotype frequency for the first 100 generations following releases of homozygotes for Killer-Rescue/Cargo (G), split50cM ClvR V1 (H), and split50cM ClvR V2 (I), for different introductions and fitness costs/transgene allele. Each rectangle indicates the average Rescue/Cargo genotype frequency for the first 100 generations for the introduction and fitness cost associated with the tick marks. Thus, the box in the upper left designates a 90% introduction with a 0% fitness cost.