Phenomic screen identifies a role for the yeast lysine acetyltransferase NuA4 in the control of Bcy1 subcellular localization, glycogen biosynthesis, and mitochondrial morphology
Fig 8
Bcy1-K313Q/R mutants partially suppress glycogen accumulation of eaf1Δ cells.
(A) Cellular glycogen levels was assessed using iodine staining. The indicated strains were serially diluted on to YPD plates, grown at 30°C for 24 hours prior to exposure to iodine crystals. Darker staining represents more glycogen within the cell. Image is representative of three independent dot assays. (B) Cellular glycogen content was assessed by measuring glucose released from hydrolyzation of glycogen. Cellular glycogen was extracted and treated with amyloglucosidase to break down glycogen into glucose. Glucose was then measured directly using the Glucose Colorimetric/Fluorometric assay kit (Biovision, K606). The amount of glucose released through the hydrolyzation of glycogen in each replicate was normalized to that of the WT strain. The Y-axis is broken (horizontal dotted line) in order to best show the data. ANOVA analysis was performed with a Tukey’s multiple comparison test comparing pairs of means. * = p<0.05, n.s. = p>0.05, relevant significance bars shown. Horizontal bars in the data represents the mean.