Defects in the GINS complex increase the instability of repetitive sequences via a recombination-dependent mechanism
Fig 8
Break-induced replication contributes to the instability of repeat tracts in psf1-1 cells. (A-D) Impact of POL32 disruption. (E-H) Impact of PIF1 disruption. The 5-FOAR mutation rates were determined at 28°C for yeast cells with the indicated genotypes carrying plasmids with the analyzed sequences; the values are medians with 95% confidence intervals calculated from data for at least ten cultures of each strain; the p values for psf1-1 pol32Δ or psf1-1 pif1Δ versus psf1-1 mutants versus wild-type strains were calculated using the nonparametric Mann-Whitney U statistical test (**** p≤0.0001; *** p≤0.001; ** p≤0.01; * p≤0.05). All associated p-values are presented in the S1 Table.