Duplicate divergence of two bacterial small heat shock proteins reduces the demand for Hsp70 in refolding of substrates
Fig 3
IbpA small heat shock proteins stably bind aggregated substrates.
(A) Experimental scheme. Heat-activated sHsps (44°C, 5 min) were applied onto BLI sensor with immobilized aggregated luciferase and subsequently dissociated in the plain buffer. (B) Association and dissociation curves. sHsps were used in 5 μM concentration, IbpABEc stoichiometry as in Fig 2A.