Ataxin2 functions via CrebA to mediate Huntingtin toxicity in circadian clock neurons
Fig 5
Atx2 knockdown decreases Htt and mHtt levels while Atx2 or Atx2-dPAM overexpression does not affect Htt or mHtt levels prior to aggregation formation.
A. GFP Intensity in the sLNv for flies expressing HttQ25 (Q25) or HttQ46 (Q46) in a TRiP RNAi library control background (TRiP Ctrl#2) and expressing Atx2 RNAi lines (Atx2 TRiP #2) at age day 5 is quantified and shown. GFP Intensity in the sLNv without aggregates formed for flies expressing HttQ25 (Q25) or HttQ46 (Q46) in a wild-type control background (Ctrl) and expressing UAS-Atx2 and UAS-dPAM at age day 2 is quantified and shown (n = 5–38; *p<0.05, **p<0.01, ***:p<0.005). B. Representative images of LNvs (sLNv and lLNv) expressing HttQ46-eGFP at age day 2 are shown in wild-type control background (Ctrl) and overexpressing Atx2 or Atx2 lacking the PAM domain (UAS-dPAM). Blue arrowheads indicate lLNvs. Blue circles label sLNvs without aggregates. Orange dash circles label sLNvs with nuclear accumulation/aggregation of HttQ46 (which were not used for GFP intensity quantification).