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Eiger/TNFα-mediated Dilp8 and ROS production coordinate intra-organ growth in Drosophila

Fig 3

Eiger expression in slow-growing cell populations relies on Dmp53 activity.

(A-C) In situ hybridization to visualize eiger transcript levels in wing discs from individuals expressing GFP (A), RACS (B) or dmycRNAi (C) under the control of ci-Gal4. (D) qRT-PCR showing eiger mRNA levels in wing discs expressing RACS or dmycRNAi with en-Gal4. Results are expressed as fold induction relative to control wing discs. (E-F) Wing discs carrying egr-lacZ transcriptional reporter and stained to visualize GFP (green) and β-gal (red in E-F; grey in E’-F’) protein expression. dmycRNAi is expressed in anterior cells with ci-Gal4 (E) or in clones using Actin-Flipout-Gal4 (F). Note increased levels of egr-lacZ in dMyc-depleted cells. (G-H) Wing imaginal discs from controls (G) or expressing Dmp53 (H) under the control of en-Gal4; tub-Gal80ts and stained to visualize egr-lacZ (green or grey) and DAPI (blue). (I-J) qRT-PCR showing eiger mRNA levels in wing discs expressing the indicated transgenes under the control of en-Gal4 (I) or sal-Gal4;tub-Gal80ts (J). Results are expressed as fold induction with respect to control wing discs. (K) ChIP assays from larvae expressing Dmp53 (sal-Gal4/+;tub-Gal80ts/UAS.Dmp53) using anti-p53 antibodies or unrelated IgG (control) followed by qPCR for a region overlapping predicted p53-binding elements at eiger and reaper promoters. eiger intronic or 3’UTR regions were used as negative controls. Data (qPCR) are mean ± s.d.

Fig 3

doi: https://doi.org/10.1371/journal.pgen.1008133.g003