Early girl is a novel component of the Fat signaling pathway
Fig 3
elgi regulates Dachs in parallel to Fat and does not affect Dachs polarity.
(A-C) Third instar wing imaginal discs expressing en-Gal4 UAS-dcr2 UAS-GFP ex-lacZ and either UAS-elgi-RNAi (A, A’) or UAS-dachs-RNAi (UAS-d-RNAi) (B, B’) or UAS-elgi-RNAi and UAS-d-RNAi (C, C’), stained for expression of ex-lacZ (magenta), with posterior cells marked by expression of GFP (green). Dashed white line marks the A-P compartment boundary. Scale bar is 33 μm. (D, E) Horizontal apical sections of wing imaginal discs expressing en-Gal4 UAS-dcr2 D:GFP UAS-Fat without (D, D’) or with (E, E’) UAS-elgi-RNAi showing displacement of membrane localized Dachs:GFP (D:GFP) into the cytoplasm. Posterior cells are marked by expression of Dcr2 or Fat (red) Dashed white line marks the A-P compartment boundary. Scale bar is 16.5 μm. (F) Horizontal apical sections of wing imaginal discs expressing Dachs:GFP (D:GFP) in flipout clones and elgi-RNAi in the posterior compartment (marked by RFP, red). Arrows point in a proximal (P) to distal (D) direction. (1, 2) Magnified views of boxed areas in F with small arrows indicating preferential enrichment of D:GFP on the distal boundary of cells. Scale bar is 16.5 μm.