OsCNGC13 promotes seed-setting rate by facilitating pollen tube growth in stylar tissues
Fig 4
The expression of OsCNGC13 (ORF1) positively affects the plant seed-setting rate.
(A) qRT-PCR analysis of OsCNGC13 and OsCNGC13-D in WT, sss1-D, and heterozygous plants with the 5F/5R primer pair shown in Fig 2D. Note that the expression of OsCNGC13 and OsCNGC13-D in the heterozygous plants was significantly reduced. (B and C) qRT-PCR analysis of OsCNGC13 and OsCNGC13-D in WT, sss1-D and heterozygous plants with allele-specific primers. Unequal reduction of expression of OsCNGC13 and OsCNGC13-D is observed in the heterozygous plant as normalized to WT (B) and sss1-D (C). (D) Comparison of mature panicles of wild type Kitaake and two independent lines (-1 and -2) of pOsCNGC13::OsCNGC13-D. Scale bars, 5 cm. (E and F) The seed-setting rate (E) and the percentages of ovules with pollen tube (F) in Kitaake and the transgenic plants. Data in E are means ± SD (n > 5). Data in F are means ± SD from 3 replicates with > 30 pistils observed per replicate. Different letters indicate a significant difference at P < 0.01 by the Student’s t-test. (G) qRT-PCR analysis of OsCNGC13 and OsCNGC13-D in Kitaake and the transgenic plants. The primer pairs used in A (5F/5R for both OsCNGC13 and OsCNGC13-D), B (6F/6R specific for OsCNGC13), C (7F/7R specific for OsCNGC13-D), G (6F/6R and 7F/7R) are shown in Fig 2D. Data are means ± SD (n = 3). (H) Positive correlation between the seed-setting rates and expression levels of OsCNGC13 in several RNAi transgenic lines. (I) Positive correlation between the seed-setting rates and the percentages of ovules with pollen tube in several RNAi transgenic lines. r value is based on two-tailed Pearson correlation analyses.