A Gap Junction Protein, Inx2, Modulates Calcium Flux to Specify Border Cell Fate during Drosophila oogenesis
Fig 4
Inx2 regulates Domeless internalization by modulating distribution of Shibire.
(A-B): Maximum intensity projected images depict localization of the Dome:GFP vesicles in the follicle cells in control (A) and inx2RNAi (B). Anti-Armadillo staining (Magenta) marks the outline of respective egg chamber. White arrowheads indicate vesicles localized to apical membrane. Yellow arrowheads mark cytoplasmic vesicles. (C): Histogram comparing the apical fraction of Dome:GFP vesicles. (D-F): Stage 9–10 egg chambers of indicated genotypes stained with anti-Armadillo (Red) and DAPI (Blue). Inset depicts the number of border cell nuclei. (G): Quantification of border cell nuclei in the migrating cluster for genotype represented in (D-F). Note the enhancement of Inx2 depletion phenotype in Shibire heterozygous background. (H-K): Mosaic analysis employing Actin<Flipout >Gal4 resulting in overexpression GFP alone (H-I) and GFP cum inx2RNAi (J-K). Follicle cells overexpressing clones are outlined in white. GFP (Green) (H, J) and anti-Dynamin (Red) (I, K). Note the change in the level and distribution of Shibire (Dynamin) protein in clones over expressing inx2RNAi construct compared to nearby wild-type cells and the control clone in (I). Error bar represents Standard Error of Mean. ‘n’ indicates number of egg chambers analyzed. *** p-value <0.001.