Elevated Fibroblast Growth Factor Signaling Is Critical for the Pathogenesis of the Dwarfism in Evc2/Limbin Mutant Mice
Fig 5
Tibia ex vivo culture indicates elevated endogenous FGF signaling in Evc2 mutant growth plates.
E16.5 control or Evc2 mutant tibiae were cultured with 20 μM (A) or 40 μM (B) U0126, or with 10 μM (C) or 20 μM (D) SU5402 for 7 days. Growth rates were calculated as the tibia length at D7 relative to D0 (n = 6, * p<0.05, ** p<0.01, # p>0.2). (E) H&E staining of tibiae after ex vivo culture. (NT, no treatment; T, treatment). Pictures show the proximal half of tibiae, with brackets pointing to drastic differences in the height of the hypertrophic chondrocyte zone among samples. Scale bars: 200 μm for (E).