TGFβ and BMP Dependent Cell Fate Changes Due to Loss of Filamin B Produces Disc Degeneration and Progressive Vertebral Fusions
Fig 6
TGFβ signaling increased in absence of FLNB in vitro and in vivo.
(A) Western blot analysis of protein lysates extracted from AF tissue of P15 pups. (B-D) Western blot analysis of protein lysates extracted from cultured primary mouse chondrocytes. (B) P-Smad3 and p-ERK levels are significantly increased both with and without TGFβ-1 (5ng/mL) stimulation in mutant cells whereas total Smad3 levels are unchanged, N = 6. (C) P-Smad2 levels are significantly increased both with and without TGFβ-1 stimulation in mutant cells, N = 6. (D) P-ERK levels are significantly increased endogenously as well as after 10 minutes of TGFβ-1 stimulation whereas total ERK levels remain unchanged, N = 6.