A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5’-3’ Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe
Fig 4
Dhp1 contributes to heterochromatin spreading.
(A) Insertion of the cenH nucleation site adjacent to the ade6+ reporter gene in an ectopic euchromatic locus (ura4+) will result in the silencing of the reporter gene through heterochromatin spreading. Gray circles: H3K9me. (B) Above, representative images of grid-based assays used to calculate the percentage of cells exhibiting distinct ade6+ expression on low adenine medium. Below: 100% stacked column graph represents the observed phenotypes of these colonies. (C) H3K9me2 qChIP shows relative enrichment at the ade6+ reporter gene and its surrounding regions normalized to leu1+. The genomic positions for oligos used for qPCR are indicated by gray boxes. * p ≤ 0.05 as determined by Student’s t test comparing the indicated sample values with wt values.