Reciprocal Effects on Neurocognitive and Metabolic Phenotypes in Mouse Models of 16p11.2 Deletion and Duplication Syndromes
Fig 5
Analysis of weight and adiposity with the standard and high-fat diets.
(A-D) Weight, adiposity and body length of the Del-Dup littermates when fed a standard diet. (A) Evolution of the body weight (g) of adult animals. (B) Body fat percentage of 20-week-old animals measured by qNMR. (C) Body length (distance from the snout to the tail base) of 20-week-old animals. (D) Adult male Del/+ (21.9 grams), +/+ (31 g), Del/Dup (33.9 g) and Dup/+ (35.8 g) littermates at 24 weeks of age. The Del/+ mice were shorter and showed a marked lack of epididymal adipose tissues (white arrows). (E) Body mass data measured over time (g). (F-I) Metabolic and endocrinological analysis of separate Del/+ and Dup/+ cohorts on the C57BL/6N background put on the high-fat diet at 5 weeks of age. (F) Body fat percentage of 13-week old animals measured by qNMR. (G) Energy expenditure (Kcal/h per kg^0.75 of body weight) during 12-h dark and 12-h light phases. (H) Intraperitoneal glucose tolerance test. Blood glucose (mg/dl) and glucose area under the curve (AUC) index (min × mg / dl × 0.001) (I) Endocrinological analysis. Blood insulin (μg/l), leptin (ng/ml) and adiponectin (μg/ml) levels. The data are presented as the mean + SEM. (A, E) Repeated Measures ANOVA “genotype” analysis, Tukey’s test. (B) Kruskal-Wallis analysis, Mann-Whitney U-test. (C) One-way ANOVA analysis, Tukey’s test. (F-I) Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 vs wt and # P < 0.05 vs all other groups.