Cognitive Function Related to the Sirh11/Zcchc16 Gene Acquired from an LTR Retrotransposon in Eutherians
Fig 2
Expression profile of mouse Sirh11/Zcchc16 mRNA.
(A) Exon-intron structure of the Sirh11/Zcchc16 gene. The full-length cDNA sequence was identified by 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) experiments using brain RNA at 8 w. The Sirh11/Zcchc16 gene consists of 7 exons and the protein coding sequence is in exon 7. The identified cDNA sequence corresponds to Genbank Accession No. NM_001033795.4. The white and black boxes represent the exons and ORF, respectively. (B) Sirh11 expression in fetuses and adults. qRT-PCR analyses were carried out using C57BL/6J cDNA from various tissues and organs at d14.5 and 8 w. The black and white bars indicate males and females, respectively. Data represents the mean ± S. D. (N = 3 each). Sirh11/Zcchc16 expression was observed in the brain, testis, ovary and kidney, but not in the placenta. qRT-PCR primers (F9R9) were designed in the 3’ UTR region of Sirh11/Zcchc16 mRNA (see arrows in Fig 2A). (C) Overexpression of the Sirh11/Zcchc16 transcript without any ORF in KO mice. The graph shows the Sirh11/Zcchc16 expression levels at 8 weeks of age using F9R9 (3’ UTR) primer sets relative to Actb mRNA in the male brain, kidney and testis. The white and black bars represent WT and KO, respectively. Each data point represents the mean ± S. D. (N = 4 each). The asterisks indicate significant differences between the WT and KO mice (**: p < 0.01).