The Escherichia coli Small Protein MntS and Exporter MntP Optimize the Intracellular Concentration of Manganese
Fig 4
MntS-overproducing cells are manganese sensitive.
Cells were pre-cultured in aerobic LB medium and then diluted at time zero into fresh LB/arabinose medium containing 0.5 mM MnCl2. Strains were MG1655 (WT) and JEM913 (Δfur) harboring empty vector (pBAD24) or pMntS (pLW112, mntS driven by the araBAD promoter). The data are representative of at least three independent experiments.