A Nitric Oxide Regulated Small RNA Controls Expression of Genes Involved in Redox Homeostasis in Bacillus subtilis
Figure 10
Targeted degradation of the sucCD mRNA upon induction of RoxS expression.
(A) Predicted base pairing between RoxS and the sucCD mRNA by TargetRNA2 (http://cs.wellesley.edu/~btjaden/TargetRNA2/). The CRR regions 2 and 3 of RoxS are shown in green. The Shine Dalgarno (SD) sequence and AUG initiation codon (start) of sucC are indicated in red. (B), (C), (D) Northern blots from Fig. 5 reprobed for the sucCD mRNA with an oligo specific for the sucD portion of the bicistronic transcript (CC1408; S4 Table). (E) Quantification of RoxS and sucCD in Northern of strains containing pDG-Ptet-roxS. Legend as in Fig. 5D. The traces are the average of two experiments, with standard errors as shown.