ZTF-8 Interacts with the 9-1-1 Complex and Is Required for DNA Damage Response and Double-Strand Break Repair in the C. elegans Germline
Figure 1
ZTF-8 is a conserved protein required for normal brood size.
A. Schematic representation of the C. elegans ZTF-8 protein and predicted related proteins in T.porphyreolophus, S.Salar, N.vectensis, X.tropicalis, H.sapiens and M.musculus, indicating the region deleted in the tm2176 mutant allele. Percent similarity and identity (S and I) compared to ZTF-8 are indicated in parentheses. Protein names and/or accession numbers are indicated. B. Western blot analysis comparing wild type and ztf-8(tm2176) mutant lysates probed with N-terminal anti-ZTF-8 and anti-histone deacetylase 1 (HDA-1; loading control) antibodies. C. Plate phenotypes of ztf-8 mutants. Brood size, embryonic (shown as % hatching) or larval (% adults) survivals are scored among the progeny of worms of the indicated genotypes. Error bars represent standard error of the mean. n = 24 for each genotype. Asterisks indicate statistically significant reduction compared to wild type (P<0.0001 by the two-tailed Mann-Whitney test, 95% C.I.).