daf-31 Encodes the Catalytic Subunit of N Alpha-Acetyltransferase that Regulates Caenorhabditis elegans Development, Metabolism and Adult Lifespan

The Caenorhabditis elegans dauer larva is a facultative state of diapause. Mutations affecting dauer signal transduction and morphogenesis have been reported. Of these, most that result in constitutive formation of dauer larvae are temperature-sensitive (ts). The daf-31 mutant was isolated in genetic screens looking for novel and underrepresented classes of mutants that form dauer and dauer-like larvae non-conditionally. Dauer-like larvae are arrested in development and have some, but not all, of the normal dauer characteristics. We show here that daf-31 mutants form dauer-like larvae under starvation conditions but are sensitive to SDS treatment. Moreover, metabolism is shifted to fat accumulation in daf-31 mutants. We cloned the daf-31 gene and it encodes an ortholog of the arrest-defective-1 protein (ARD1) that is the catalytic subunit of the major N alpha-acetyltransferase (NatA). A daf-31 promoter::GFP reporter gene indicates daf-31 is expressed in multiple tissues including neurons, pharynx, intestine and hypodermal cells. Interestingly, overexpression of daf-31 enhances the longevity phenotype of daf-2 mutants, which is dependent on the forkhead transcription factor (FOXO) DAF-16. We demonstrate that overexpression of daf-31 stimulates the transcriptional activity of DAF-16 without influencing its subcellular localization. These data reveal an essential role of NatA in controlling C. elegans life history and also a novel interaction between ARD1 and FOXO transcription factors, which may contribute to understanding the function of ARD1 in mammals.


Introduction
Animal development is a complex process that involves hierarchical gene regulatory networks and is influenced by environmental conditions. When food is abundant, the postembryonic development of C. elegans consists of four larval stages (L1-L4) and the adult. During the L1 stage, environmental factors determine whether C. elegans molts to an L2 larva or a pre-dauer L2d larva [1]. At least three environmental cues have been defined: food supply, temperature, and a constitutively secreted dauer-inducing pheromone that signals population density [2]. The L2 larva is developmentally committed to continued growth, whereas the L2d larva can molt to a dauer larva if food is scarce and the animals are overcrowded, or to an L3 larva should conditions improve.
Mutations affecting dauer larval development include dauerdefective (daf-d) mutations that prevent entry into the dauer stage, and dauer-constitutive (daf-c) mutations that mandate entry into the dauer stage [2]. Based on epistatic relationships between daf-c and daf-d mutations, more than twenty genes controlling dauer formation have been ordered in a genetic pathway [2] representing generation of the pheromone signal [3], response by chemosensory neurons [4,5] and transduction of the signal to other cells. Three functionally overlapping neural pathways control the developmental response to environmental cues. They involve DAF-7/TGF-ß [6,7], DAF-11/cyclic GMP [8], and DAF-2/insulin-like [9,10] pathways, which relay the environmental signals to a nuclear hormone receptor, DAF-12 [11], to control dauer versus non-dauer morphogenesis.
Mutations in two genes, daf-9 and daf-15, lead to nonconditional formation of detergent-sensitive dauer-like larvae [12]. These mutants form dauer larvae constitutively and display some characteristics of dauer larvae formed under starvation, such as a high density of intestinal and hypodermal storage granules. daf-9 encodes a cytochrome P450 related to those involved in the biosynthesis of steroid hormones in mammals [13,14]; it was found to specify a step in the biosynthetic pathway for a DAF-12 steroid ligand called dafachronic acid [15][16][17]. daf-15 encodes the C. elegans ortholog of Raptor [18] that is proposed to interact with C. elegans target-of-rapamycin kinase (LET-363/CeTOR) to control C. elegans larval development [18]. Both daf-9 and daf-15 also regulate fat metabolism and adult lifespan [13,14,18].
The dauer-like mutants represent a mutant class distinct from the previously defined daf-c and daf-d mutants. Unlike most daf-c mutants, the dauer-like mutants are not ts, and they do not complete dauer morphogenesis. The daf-d genes such as daf-12 have non-conditional alleles and fail to respond to pheromone [1], but unlike the dauer-like mutants they can execute non-dauer development. The dauer-like mutants define a third class of mutants, one in which the animals are incapable of executing either complete dauer or non-dauer development.
The daf-31 mutant was isolated in genetic screens to identify genes similar to daf-9 and daf-15 [17]. The overall aim of the present study was to clone the daf-31 gene and characterize the DAF-31 function. Our genetic epistasis analysis suggests daf-31 functions downstream of or in parallel to daf-3, daf-12 and daf-16 dauer-defective genes, and acts upstream of or in parallel to daf-15/raptor. We cloned the daf-31 gene by positional cloning and showed that it encodes an ortholog of arrest-defective-1 protein (ARD1), the catalytic subunit of the major N alpha-acetyltransferase (NatA). Moreover, our data reveal that daf-31 has an essential role in controlling C. elegans larval development, metabolism and adult longevity.

daf-31 mutations cause developmental larval arrest
Entry into the dauer stage is determined by the pheromone/ food ratio, with high pheromone and low food supply favoring dauer formation [2]. Dauer larva is considered as an alternative L3 larval stage. Compared to L3 larva ( Figure 1A), the dauer larva has a constricted pharynx ( Figure 1B) and a special cuticle with

Author Summary
The development of a living organism is influenced by the environmental conditions such as nutrient availability. Under starvation conditions, the C. elegans larvae will enter a special developmental stage called dauer larva. An insulin-like signaling pathway controls dauer formation as well as adult lifespan by inhibiting the activity of FOXO transcription factor DAF-16 that regulates expression of stress-resistant genes. Here we isolate a new gene called daf-31; this gene encodes a protein that regulates C. elegans larval development, metabolism and adult lifespan. This protein has been found in other species to be part of an enzyme that functions to modify other proteins. We show that overexpression of our newly discovered protein stimulates the transcriptional activity of DAF-16. Interestingly, abnormal regulation of human proteins similar to DAF-31 results in tumor formation. It is known that human FOXO proteins prevent tumorigenesis. Therefore, it is possible that abnormal DAF-31 activity may lead to tumor growth by reducing DAF-16 activity. Thus, the present study may not only contribute to understanding the role of a universal enzyme in controlling development, metabolism and lifespan in other organisms besides worms but may also shed light on the mechanisms of tumorigenesis in humans. . Arrows indicate isthmus of pharynx in panels A, B and D. Arrowheads indicate dauer alae in panels C and E. N2 and unc-24daf-31/nT1 animals were grown on NG agar plates at 20uC. Unc dauer larvae (unc-24daf-31) were identified after animals were starved. (F-H) Representative pictures showing fat accumulation detected by Sudan Black B in daf-2 mutants (F), N2 (G) and daf-31 mutants (H). N2, daf-2(e1370) and daf-31(m655)IV/nT1[unc-?(n754) let-?](IV;V) synchronized L1 larvae were placed on NG agar plates, incubated at 20uC until they entered L3 or dauer-like stages, then collected for staining. Scale bars: 10 mm. doi:10.1371/journal.pgen.1004699.g001 DAF-31/ARD1 Regulates C. elegans Life History dauer alae ( Figure 1C). In the presence of dauer-inducing pheromones, daf-31 mutants cannot form SDS-resistant dauer larvae [17]. In order to determine whether daf-31 mutants enter the dauer stage in response to starvation, we examined the progeny of strain unc-24daf-31/nT1 under starvation conditions and observed uncoordinated (Unc) dauer larvae. These dauer larvae showed normal dauer features, such as a dark body, fully constricted pharynx (Figure 1D), and a cuticle with dauer alae ( Figure 1E). However, daf-31 dauer larvae were not SDS-resistant like normal dauer larvae. Furthermore, daf-31 dauer larvae could not resume development when food was provided, dying shortly thereafter. Therefore, daf-31 mutants could not complete dauer morphogenesis under starved conditions, and those incomplete dauer larvae could not finish reproductive development after food was provided.
Fat accumulation is one characteristic of C. elegans dauer larvae. We examined fat accumulation in daf-31 homozygous mutants using Sudan Black B staining. As shown in Figure 1F, daf-2 mutant dauer larvae accumulate fat as described previously [9]. The daf-31 mutant worms also accumulate more fat droplets than wild-type worms and fat droplets in the daf-31 mutants are larger than those in wild-type worms ( Figure 1G and 1H). To confirm this phenotype, Nile red was used to stain fixed worms; this approach has been reported to reliably detect fat droplets in C. elegans [19]. Similar to Sudan Black staining, Nile red also detected fat accumulation in daf-31 mutant worms ( Figure S1). Therefore, daf-31 mutants shift metabolism to fat accumulation. daf-31 is epistatic to daf-d genes but acts upstream of or in parallel to daf-15/raptor To position daf-31 in the dauer formation pathway, we examined the epistatic relationship between daf-31 and daf-d genes including daf-3, daf-12 and daf-16. The daf-31 mutation is epistatic to all three daf-d mutations as judged by the ratio of progeny (1:2:1) ( Table 1). For the epistasis analysis with daf-16, the ratio of progeny is 1:2 because nT1 homozygous animals are lethal. We repeated the epistasis analysis of daf-31 and daf-12 by using the daf-12(rh61rh411) null allele [11] and obtained a similar result (Table 1). These epistatic relationships suggest that daf-31 functions downstream of or in parallel to daf-3, daf-12 and daf-16 in dauer formation.
To examine the epistatic relationship of daf-31 and daf-15, we injected dsRNA of daf-15 into unc-24daf-31/nT1 young adult worms. Wild-type (N2) animals were treated equally and used as controls. We examined the phenotype of progeny reproduced at various time periods after injection. The progeny reproduced between seven and eighteen hours after injection arrested develop-ment at a dauer-like stage three days after egg lay ( Table 2). These dauer-like animals have a similar phenotype to daf-15 mutants. Thus, regarding dauer entry, it appears that the daf-15 mutation is epistatic to the daf-31 mutation. We scored the recovery of both N2 and unc-24daf-31 dauer-like animals two days after dauer-like arrest. 48% of N2 dauer-like worms remained at dauer-like stage and the rest of the animals recovered and grew to L4 larval or adult size ( Table 2). By contrast, 100% of unc-24daf-31 animals stayed at dauer-like stage ( Table 2). For unc-24daf-31 dauer-like larvae without daf-15 RNAi treatment, the majority of these animals died within five days. However, surviving animals all grew to L4 larval or adult size (n = 52). Taken together, these data indicate that daf-15 is epistatic to daf-31 as unc-24daf-31 mutants treated by daf-15 RNAi form dauer-like larvae similar to daf-15 mutants. Moreover, these two mutants have a synergistic effect on C. elegans development because no unc-24daf-31 dauer-like larvae treated by daf-15 RNAi recovered. Thus, these two genes may function in the same pathway and daf-31 is upstream of daf-15. However, the possibility that these two genes act in parallel cannot be excluded.

daf-31 encodes an ortholog of ARD1
A positional cloning strategy was used to identify the daf-31 gene on chromosome IV between unc-24 and fem-3 ( Figure S2A). daf-31 was found to lie between the physical SNP markers T09A12 and F17E9 ( Figure S2A). A genomic fragment corresponding to the K07H8.3 open reading frame fully rescued the genetic daf-31 null mutant [daf-31(m655)] phenotype, i.e. the transgenic animals did not form dauer-like larvae, but grew to fertile adults. Sequence analysis of the daf-31 gene in the mutant revealed a 393 bp deletion which removed 151 bp of promoter upstream of the ATG start codon and 242 bp of daf-31 coding region downstream of the ATG start codon, which may completely block daf-31 transcription as both the essential promoter region and the N-terminal portion of the gene were deleted ( Figure S2B). Primers were designed to flank the deletion region and PCR analysis of mutant worms' genomic DNA detected a 1,449 bp band (393 bp smaller than the wild-type band) in both homozygous and heterozygous daf-31(m655) mutant worms ( Figure S2C).
The daf-31 gene encodes the ortholog of ARD1 with a predicted molecular weight of 21.2 kDa. ARD1 is the catalytic subunit of NatA that catalyzes the acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala [20]. Amino acid sequence alignment showed that DAF-31 shares 75% identity with human ARD1, 77% identity with mouse ARD1, 72% identity with Drosophila melanogaster ARD1 and 46% identity with yeast ARD1 ( Figure S2D). Given that there is only a single mutant allele of daf-31, we used RNAi to inhibit daf-31 in the N2 background to confirm the daf-31 mutant phenotype. Inhibition of daf-31 by feeding animals with E. coli that express daf-31 dsRNA did not induce a dauer-like phenotype. From our previous work, dsRNA injection can create a stronger mutant phenotype similar to that of a genetic null mutant [18]. In vitro synthesized daf-31 dsRNA was injected into gonads of N2 young adult worms and the progeny displayed a dauer-like phenotype similar to the daf-31(m655) mutants. The starvationinduced dauer morphology of daf-31(m655) mutants, such as dauer alae formation and contrasted pharynx (described in Figure 1) could not be examined using this RNAi method. Therefore, we examined fat accumulation in daf-31 RNAi-treated animals. Similar to the daf-31(m655) mutant, daf-31 RNAitreated animals accumulated fat as detected by both Sudan Black and Nile red staining of fixed animals ( Figure S3). Based on these results, we conclude that the daf-31 mutant phenotypes described in this study most likely resulted from daf-31 mutation instead of secondary mutations in the background.

daf-31 is expressed in multiple tissues
In order to characterize the daf-31 expression pattern, we constructed a daf-31 promoter::gfp reporter construct. In N2 animals, GFP expression was detected from L1 to the adult stages in multiple tissues including the hypodermis, pharynx, intestine, and neurons ( Figure 2). To confirm the GFP expression pattern of daf-31 promoter fusion reporter, we constructed daf-31 translational fusion reporter genes in which the GFP open reading frame was fused to the full-length daf-31 genomic DNA in frame either at the N-terminus or at the C-terminus. Both translation fusion reporter genes fully rescued the dauer-like phenotypes of daf-31 mutants. However, we did not observe GFP expression in the rescued daf-31 mutant worms, a phenomenon previously reported with other GFP fusion gene mutant rescues [21]. Thus, our observations of daf-31 expression pattern were limited to the daf-31 promoter fusion, which may not represent the endogenous expression pattern of the entire daf-31 gene if enhancer elements are present in introns or in 39 untranslated sequences.

daf-31 influences C. elegans lifespan
Increased adult longevity is a phenotype associated with many dauer mutants. Since daf-31 homozygous mutants arrest development at L4 stage, we inhibited the daf-31 gene by feeding RNAi. The RNAi treatment successfully reduced daf-31 mRNA level as measured by qRT-PCR ( Figure S4). However, daf-31 RNAi treatment had no obvious effect on the lifespan of wild-type worms as the daf-31 RNAi-treated worms had similar mean and maximum lifespans as control vector RNAi-treated worms ( Figure 3A and Table S1). When RNAi-sensitive rrf-3(pk1426) mutants were treated with daf-31 RNAi, their lifespans were significantly decreased (p,0.0001, log-rank test) ( Figure 3B and Table S1). Compared to controls, the mean lifespan of rrf-3 mutants treated with daf-31 RNAi was shortened by four days ( Figure 3B and Table S1).
To test if daf-31 mutations influence the longevity phenotype of daf-2 mutants, we treated the rrf-3(pk1426);daf-2(e1370) mutant with daf-31 RNAi. The mean lifespan of daf-31 RNAi-treated rrf-3;daf-2 mutants was five days shorter compared to that of control animals (p = 0.0005, log-rank test) ( Figure 3C and Table S1). Thus, inhibition of daf-31 partially suppressed the longevity phenotype of daf-2 mutants. Based on this result, we postulate that overexpression of daf-31 may further increase the lifespan of daf-2 mutants.
To test this, daf-2(e1370) and daf-16(mgDf47);daf-2(e1370) mutants overexpressing daf-31 were constructed. The overexpression of daf-31 was confirmed by qRT-PCR ( Figure S4). As shown in Figure 3D, daf-31 overexpression increased the lifespan of daf-2 mutant worms (p,0.0001, log-rank test) ( Figure 3D and Table  S1). The mean lifespan was increased by eight days and the maximum lifespan was extended by seven days ( Figure 3D and Table S1). This increased lifespan was due to daf-31 overexpression as daf-31 RNAi treatment completely abrogated it ( Figure 3E and Table S1). Interestingly, daf-31 overexpression failed to extend the lifespan of daf-16;daf-2 double mutants ( Figure 3F and Table S1), indicating that DAF-16 is required for daf-31 overexpression to enhance the daf-2 longevity phenotype. We also measured the lifespan of N2 animals overexpressing the daf-31 gene. As shown in Figure S5 and Table S1, daf-31 overexpression did not extend the lifespan of N2 worms. In fact, it slightly decreased the lifespan of N2 worms. Finally, to confirm daf-31 functions through daf-16 in C. elegans lifespan regulation, we tested if daf-31 RNAi can further decrease the lifespan of RNAi-sensitive daf-16 mutants (daf-16;rrf-3). We found daf-31 RNAi had no obvious effect on the lifespan of daf-16;rrf-3 mutants ( Figure S6 and Table S1).

daf-31 overexpression stimulates daf-16 transcriptional activity
The forkhead transcription factor DAF-16/FOXO controls the transcription of an array of genes essential for lifespan extension and oxidative stress resistance including the antioxidant enzyme superoxide dismutase (sod)-3 gene and beta-carotene 15,159monooxygenase gene (bcmo-2) [22]. We used qRT-PCR to measure the expression level of sod-3 and bcmo-2 in daf-31 overexpression strains and control animals. As shown in Figure 4A, the expression level of sod-3 was significantly increased when daf-31 was overexpressed in the N2 background and in daf-2 mutants. Similar to sod-3, the expression of bcmo-2 is also significantly increased when daf-31 is overexpressed in the daf-2 mutant background ( Figure 4B). Taken together, these data indicate overexpression of daf-31 stimulates the transcriptional activity of DAF-16.
Reduction of daf-2 insulin-like signaling activity increases C. elegans lifespan by promoting nuclear localization of DAF-16  [23,24]. We crossed the DAF-16::GFP reporter gene into daf-31 overexpressing animals to examine if daf-31 overexpression influences the subcellular localization of DAF-16. We found the percentage of animals showing DAF-16 nuclear localization was not significantly different between daf-31 overexpressing animals and control worms ( Figure 4C and D).

Discussion
We demonstrated that daf-31 mutants form dauer-like larvae that share some characteristics of wild-type dauer larvae such as fat accumulation. Many daf genes, especially those from the insulin-like signaling pathway, are involved in the regulation of lifespan [27]. Mutations in daf-2, which encodes an insulin/IGF-1 receptor [9], convey a temperature-sensitive Daf-c phenotype, and the adults live twice as long as wild-type animals [9,28,29]. Mutations in some genes downstream of daf-2, such as age-1 and pdk-1, also extend lifespan [30,31]. Conversely, mutations in other downstream genes, including daf-18 and daf-16, shorten lifespan [32][33][34].
We examined whether daf-31 is also involved in aging and found that daf-31 partially mediates the effect of reduced daf-2/ IGF signaling pathway on C. elegans lifespan. Moreover, overexpression of daf-31 enhances the longevity phenotype of daf-2 mutants depending on the activity of DAF-16. Supporting this lifespan data, the expression levels of sod-3 and bcmo-2, the transcriptional targets of the DAF-16 FOXO3 transcription factor, are up-regulated in the daf-31 overexpression strains. Thus, it is reasonable to argue that DAF-31 regulates C. elegans lifespan by influencing DAF-16 transcriptional activity and daf-31 overexpression stimulates DAF-16 activity. Indeed both DAF-31 and DAF-16 are expressed in neurons and intestine, two major tissues essential for regulation of C. elegans lifespan by the DAF-2/IGF signaling pathway [35][36][37]. However, overexpression of daf-31 has no influence on the subcellular localization of DAF-16. It is consistent with the lifespan data that overexpression of daf-31 does not increase the lifespan of N2 animals. Thus, overexpression of DAF-31 only extends C. elegans lifespan in the daf-2 mutant background in which DAF-16 has entered the nucleus due to inhibition of the IGF signaling.
Previous studies show that the stress-resistance phenotype can be uncoupled from the longevity phenotype [38]. Indeed, although daf-31 overexpression further increases the long-lived lifespan of daf-2 mutants, it has no effect on the thermotolerance of daf-2 mutants. Interestingly, daf-31 overexpression increases the reproduction of both wild-type animals and daf-2 mutants, which is not dependent on DAF-16, suggesting DAF-31 functions through DAF-16 for lifespan regulation but not for reproduction. Since DAF-16 is required for the stress resistance of daf-2 mutants, it is likely that daf-31 overexpression extends the daf-2 mutant lifespan through DAF-16-dependent mechanisms other than increasing stress-resistance. DAF-31 is found in multiple tissues including neurons. It is known that many C. elegans neurons are refractory to RNAi treatment in wild-type background [39]. It is possible that neuronal DAF-31 activity is more important for lifespan regulation because daf-31 RNAi treatment only shows influence on lifespan of RNAi-sensitive mutants. Supporting this assumption, it has been reported that daf-16/FOXO activity in neurons accounted for only 5-20% of the lifespan extension seen in daf-2 mutants [37]. Since DAF-31 may only influence DAF-16 activity in neurons, its overexpression only increases the daf-2 mutant lifespan modestly.
We cloned the daf-31 gene and sequence analysis indicates DAF-31 is a worm ortholog of ARD1 that was first identified in yeast [40]. ARD1 is the catalytic subunit of the major NatA that transfers an acetyl group from acetyl coenzyme A to the Nterminal of nascent polypeptides. Yeast ARD1 mutants fail to enter stationary phase and sporulate during nitrogen deprivation [40]. The yeast stationary phase is comparable to C. elegans dauer stage and is essential for survival when nutrients are limited. C. elegans enters dauer stage during starvation or under high concentration of pheromone. Our data show daf-31 mutants could not complete dauer morphogenesis in response to pheromone and starvation, which indicates daf-31 is required for dauer formation. Thus, both yeast ARD1 and worm DAF-31 play an important role in the developmental switch in response to the environmental nutrient limitation. Additionally, daf-31 mutants could not grow to fertile adults in an environment with abundant food suggesting its essential role in normal development. Similar to our observation, it has been reported that loss of Ard1 is lethal for D. melanogaster and affects cell survival or proliferation, indicating ARD1 is required for D. melanogaster development [41]. In addition to developmental arrest, the daf-31 mutants shift metabolism to fat accumulation. Interestingly, yeast ARD1 mutants not only fail to enter stationary phase but also do not accumulate as much carbohydrates as wild-type yeast strains [40]. Thus, the function of ARD1 in regulating development and metabolism appears conserved from yeast to C. elegans.
N-terminal acetylation is one of the most common posttranslational protein modifications. It is estimated to occur on 50% of yeast proteins [20], 71% of D. melanogaster cytosolic proteins [20] and 84% of human proteins [42]. NatA plays the most prominent role in N-terminal acetylation. It would be interesting to know whether the pleiotropic phenotypes of ard1 mutants result from global changes of protein N-acetylation or from acetylation status of specific protein substrates. It has been reported that human ARD1 directly acetylates b-catenin and enhances its transcriptional activity [43]. We show that overexpression of DAF-31 stimulates the transcriptional activity of DAF-16. It would be interesting to examine if DAF-31 overexpression acetylates DAF-16. Alternatively, a suppressor screening of daf-31 mutants may help to identify the essential substrates of the DAF-31 acetyltransferase and contribute to understanding the mechanisms by which ARD1 influences development, metabolism and aging. Moreover, emerging evidence has revealed that abnormal regulation of ARD1 is associated with tumorigenesis and ARD1 represents a novel cancer drug target [44,45]. Identification of DAF-31 substrate proteins may uncover new therapeutic targets of cancer diseases.
To construct the daf-31 overexpressing strains, the full-length daf-31 genomic DNA, including its native 760-bp promoter and 39-UTR was cloned into pGEM-T (Promega); this construct successfully rescued the daf-31 dauer-like mutant phenotype. Multiple copies of the construct were integrated into chromosomal DNA by c-irradiation to make an N2 transgenic line overexpressing daf-31. Then the daf-31 overexpressing chromosome was introduced into both daf-2(e1370) and daf-16(mgDf47);daf-2(e1370) mutants by genetic crosses.
To make a daf-31 promoter-GFP transcriptional fusion, the 760-bp daf-31 promoter was inserted into the GFP vector pPD95.70 (a gift from Dr. Andrew Fire at Stanford University) between the PstI and BamHI sites. The construct was injected into N2 adults at a concentration of 100 mg/ml. pRF4, which encodes a mutant collagen and induces a dominant roller (Rol) phenotype, was co-injected at the same concentration as a transformation marker. Rol animals were selected from the F2 generation and used to establish stable transgenic lines.

Fat staining
To stain fat using Sudan Black B, N2, daf-2(e1370) and daf-31(m655)IV/nT1[unc-?(n754) let-?](IV;V) synchronized L1 larvae were placed on NG agar plates, incubated at 20uC until they entered L3 or L4 stages, collected and washed two to three times with M9 buffer. Paraformaldehyde stock solution (10%) was added to a final concentration of 1%. The samples were frozen in dry ice/ethanol and then thawed under a stream of warm water. After a total of three freeze-thaw cycles, the worms were stained with Sudan Black B as described by Kimura et al. [9].
Nile red staining of fixed worms was performed as described by Pino et al. [47]. Worm samples were collected and washed twice with M9 buffer. After the final wash, worms were fixed in 40% isopropanol at room temperature for three minutes. The fixed worms were stained in Nile red/isopropanol solution for 30 minutes at room temperature with gentle rocking. The stained worms were washed once with 1 ml M9 buffer and mounted on a 2% agarose pad for microscopy under the fluorescence channel. In order to compare the fat content in different strains, the pictures were taken at the same camera setting under 206 magnification.

RNAi
Part of the daf-31 coding region was amplified from C. elegans genomic DNA using primers 59-CGG GAT CCA TTC GTT GTG CTC GCG TG -39 and 59-CCC AAG CTT GCA GTG GTA TAG GCC TC -39. The PCR products were then purified and cloned into the feeding RNAi vector L4440 (Addgene) between the BamHI and HindIII sites. The RNAi construct was transformed into E. coli HT115 (DE3) and RNAi feeding was performed as previously described [48].
To inhibit daf-15 and daf-31 genes by injection of RNAi, a 1 kb daf-15 cDNA fragment and the full-length daf-31 cDNA were cloned into pGEM-T vector (Promega), respectively. The gene identity was confirmed by sequencing. The Riboprobe Combination System-SP6/T7 (Promega) was used to transcribe RNA in vitro according to the manufacturer's protocol. Double-stranded RNA was synthesized and injected as described by Fire et al. [49].

qRT-PCR
Synchronized N2 L1 larvae were treated with either control (empty) vector or daf-31 RNAi by feeding as previously described [48]. Day 1 adult animals were collected for total RNA extraction using the Trizol kit (Zymo). Synchronized L1 larvae of daf-31 overexpressing strains were allowed to grow on OP50 food plates. Day 1 adult animals were collected for total RNA extraction using the Trizol reagent (Zymo). The first strand cDNA was synthesized using the ImProm-II reverse transcription system (Promega). SYBR green dye (Quanta) was used for qRT-PCR to measure the expression level of daf-31, sod-3 and bcmo-2 in corresponding worm samples. Reactions were performed in triplicate on an ABI Prism 7000 real-time PCR machine (Applied Biosystems). Relative-fold changes were calculated using the 2 2DDCT method. The primers used for qRT-PCR were: daf-31,

Adult lifespan, thermotolerance and reproduction
About 120-150 L4 larvae raised at 20uC were transferred to ten NG agar plates (twelve to fifteen animals per plate spread with either OP50 or RNAi food) and incubated at 25uC. The first day of adulthood is day 1 in the survival curves. During the reproductive period, adult animals were transferred daily to fresh plates. Thereafter, animals were transferred every ten days (OP50 food) or every six days (RNAi food). Animals were scored as alive, dead, or lost every other day. Animals that do not move in response to touching were scored as dead. Animals that died from causes other than aging, such as sticking to the plate walls, internal hatching or bursting in the vulval region, were scored as lost. GraphPad Prism was used for statistical analysis and generation of survival curves. For the thermotolerance experiment, day 1 adult animals were incubated at 35uC and survival was scored as described above. To measure reproduction of worms, L4 larvae growing at 20uC were transferred daily to fresh plates and the progeny were counted. Figure S1 Fat accumulation in daf-31 mutants. Nile red staining of fixed worms detects more fat droplets in daf-2(e1370) (A) and daf-31 mutants (C) than those in N2 animals (B). N2, daf-2(e1370) and daf-31(m655)IV/nT1[unc-?(n754) let-?](IV;V) synchronized L1 larvae were placed on NG agar plates, incubated at 20uC until they entered L3 or dauer-like stages, then collected for staining. Scale bars: 10 mm.  Figure S3 Fat accumulation in daf-31 RNAi-treated wild-type animals. Sudan Black staining and Nile red staining of fixed worms detect more fat droplets in daf-2(e1370) (A and D, respectively) and daf-31 mutants (C and F, respectively) than those in N2 animals (B and E, respectively). Scale bars: 10 mm. (TIF) Figure S4 daf-31 RNAi knocks down the mRNA level of daf-31. qRT-PCR shows the reduced daf-31 mRNA level in daf-31 RNAi-treated wild-type worms and increased daf-31 mRNA level in daf-31 overexpressing worms. daf-31 RNAi treatment successfully knocks down daf-31 mRNA level in daf-31 overexpressing worms. ***, P,0.001 (t-test). (TIF) Figure S5 daf-31 overexpression does not extend wild-type C. elegans lifespan. N2 worms and daf-31 overexpressing animals were grown at 20u in the presence of food. L4 hermaphrodites were picked up for lifespan experiments. The statistical analysis of lifespan data is presented in Table S1. (TIF) Figure S6 daf-31 RNAi does not influence the lifespan of RNAi-sensitive daf-16 mutants. daf-16;rrf-3 animals were fed E. coli that express daf-31 dsRNA or E. coli carrying the empty vector. The lifespan of RNAi-treated progeny were measured at 20u. (TIF) Figure S7 Thermotolerance of animals overexpressing daf-31. daf-31 overexpression does not influence the resistance of N2 worms (p = 0.2420, log-rank test) and daf-2 mutant adults to heat stress at 35uC (P = 0.4623, log-rank-test). (TIF) Figure S8 daf-31 overexpression increases reproduction. daf-31 overexpression increases the total number of progeny of N2 and daf-2 mutants, and is not dependent on DAF-16. ** P,0.01, *** P,0.0001 (t-test).