Regulation of Arabidopsis Flowering by the Histone Mark Readers MRG1/2 via Interaction with CONSTANS to Modulate FT Expression
Figure 5
MRG2 physically interacts with CO.
A. Top panel, flowering time of WT, mrg1 mrg2, co and co mrg1 mrg2, as measured by rosette leaf number at bolting, in plants grown under LD conditions. The mean value from 20 plants is shown. Error bars represent standard deviations. Bottom panel, relative expression of FT in indicated genotypes at ZT16. Values are normalized to ACTIN2. Error bars show standard deviation from three biological replicates. B. Interaction of purified His-tagged MRG2 and GST-fused CO from E. coli by pull-down assays. C. BiFC analysis of the interaction between MRG2 and CO in tobacco leaf cells. Bar = 50 µm. D. Co-IP detection of MRG2 and CO interaction in planta. Total protein extracts from plants expressing YFP-MRG2 alone, MYC-CO alone, or both YFP-MRG2 and MYC-CO were immunoprecipitated with anti-MYC affinity beads and the resulting fractions analyzed by western blot using anti-GFP antibodies (top panel) or HRP-conjugated anti-MYC monoclonal antibodies (bottom panel).