The Nesprin Family Member ANC-1 Regulates Synapse Formation and Axon Termination by Functioning in a Pathway with RPM-1 and β-Catenin
Figure 3
anc-1 and bar-1 function in the same genetic pathway to regulate synapse formation.
(A) Epifluorescent microscopy was used to visualize presynaptic terminals labeled using the transgene juIs1 for the indicated genotypes. Arrows highlight regions lacking presynaptic terminals represented by SNB-1::GFP puncta. Arrowheads note abnormal aggregation of presynaptic terminals. Scale bar is 10 µm. (B) Quantitation of the average number of SNB-1::GFP puncta per 100 µm of dorsal cord for the indicated genotypes. Analysis was done on young adults grown at 25°C. Significance was determined using an unpaired Student's t test; error bars represent the standard error of the mean. ***P<0.001, ns = not significant.