Genome-Wide Profiling of Yeast DNA:RNA Hybrid Prone Sites with DRIP-Chip
Figure 2
DNA:RNA hybrids are enriched at protein-encoding genes and retrotransposons of higher transcriptional frequency.
(A) Average gene profile of DNA:RNA hybrids at ORFs enriched for DNA:RNA hybrids under wild type conditions. (B–D) CHROMATRA plots of DNA:RNA hybrid distribution along genes sorted by their length (B), grouped into five transcriptional frequency categories as per [69]) (C) or grouped into four GC content categories (D). Genes were aligned by their TSSs. (E) The average DNA:RNA hybrid score at Ty1, Ty2, Ty3, Ty4 and Ty5 retrotransposons in the left panel shows higher enrichment at Ty1 and Ty2 retrotransposons. The average profile of DNA:RNA hybrids at all retrotransposons under wild type conditions is shown in the right panel.