C/EBPα Is Required for Long-Term Self-Renewal and Lineage Priming of Hematopoietic Stem Cells and for the Maintenance of Epigenetic Configurations in Multipotent Progenitors
Figure 5
ChIP-seq analysis of C/EBPα binding in LSK cells.
(A) Selected examples of ChIP-seq coverage of C/EBPα peaks in LSK cells. (B) ChIP-qPCR for IgG and C/EBPα. Enrichment was determined based on a positive vs. negative primer pair. (C) The distance from all C/EBPα peaks, the 25% highest C/EBPα peaks, or a random set, to the nearest TSS. The dashed lines represent the median. (D) Enriched TF binding motifs at C/EBPα-bound regions. P: P-value, Af: affinity score. See also Table S3. (E) Gene set enrichment analysis of CebpaΔ/Δ HSCs compared to Cebpafl/fl HSCs for PU.1 and EGR2 target genes. (F) GREAT analysis of C/EBPα-bound regions. See also Table S4. (G) Expression of C/EBPα targeted GO categories in CebpaΔ/Δ and Cebpafl/fl HSCs. (H) Relative expression of C/EBPα targeted GO categories during normal myeloid differentiation. (I) Overlap of C/EBPα bound regions in LSKs and GMPs.