Activin Signaling Targeted by Insulin/dFOXO Regulates Aging and Muscle Proteostasis in Drosophila
Figure 5
Activin regulates muscle autophagy.
(A) Autophagosomes indicated using an Atg8a-Cherry reporter in Activin RNAi flies or babo over-expressing (babo-Act) flies. 3-day old females. (B) Quantification of autophagosomes for Figure 5A (n = 20). (C) mRNA expression of autophagy genes (Atg1, Atg5, Atg6 and Atg8a) in aging muscle, at 10 days, 25 days and 45 days. (D) Phosphorylation of Smox in muscle increases with age. The average band intensity from three independent experiments was quantified using Image Lab software. (E) Inactivation of daw in muscle up-regulates autophagy gene expression. (F) RNAi against Smox in muscle up-regulates autophagy gene expression. (G) Constitutive activation of babo (babo-Act) in muscle inhibits autophagy gene expression. Asterisk indicates significant difference between treatment and control (p<0.05).