Genome-Wide High-Resolution Mapping of UV-Induced Mitotic Recombination Events in Saccharomyces cerevisiae
Figure 7
System to monitor crossovers on chromosome XII.
A diploid was constructed with heterozygous markers immediately centromere-proximal to the ribosomal RNA (rRNA) gene clusters (HYG), within the rRNA gene cluster (TRP1), and immediately centromere-distal to the cluster (URA3) [34]. G1-synchronized cells were treated with UV, plated on solid medium and grown non-selectively. The resulting colonies were replica-plated to medium lacking uracil, tryptophan, or containing hygromycin as described in the text. A. Sectoring pattern expected for a crossover centromere-proximal to the HYG marker. B. Sectoring pattern expected for a crossover within the rRNA gene cluster centromere-proximal to TRP1. C. Sectoring pattern expected for a crossover within the rRNA gene cluster centromere-distal to TRP1.