Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I
Figure 6
Role of the CID in monopolar attachment of sister kinetochores during meiosis I.
Kinetochores are composed of three layers namely inner (yellow oval), central (green rectangle) and outer (orange rectangle). They are assembled at centromeres on sister chromatids (in blue). The outer kinetochore interacts with the growing ends of microtubules (in green). In wild type cells, monopolar attachment is achieved by interaction of the Csm1-Interaction Domain (purple) in Dsn1 (a subunit of the central kinetochore MIND complex) with the globular domain of Csm1 (red), a monopolin (V-shaped structure in blue) subunit. In cells expressing a form of Dsn1 lacking the Csm1-Interaction Domain, monopolins fail to bind to kinetochores and consequently the sister kinetochores are bi-oriented.