Odoriferous Defensive Stink Gland Transcriptome to Identify Novel Genes Necessary for Quinone Synthesis in the Red Flour Beetle, Tribolium castaneum
Figure 11
Phenol oxidase (PO) activity assays of wild-type and novel quinone-less gene RNAi knock-downs in females.
The Y-axis indicates the square root of PO Vmax, red boxes are boxplots, green lines represent the mean value, the gray line represents the grand mean, while the X-axis presents wild-type, control injections, and different RNAi-knock-downs (N = 12–25). Buffer: buffer-injection control; EGFP: dsEGFP-injection control; GT39: Tcas-ql VTGl; GT62: Tcas-ql ARSB; GT63: Tcas-ql MRP. The asterisks (*) mark the t-test results comparing to wild-type: ***: p<0.001; **: 0.001<p<0.01. Buffer- and EGFP-injected controls were not significantly different from wild-type.