Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest
Figure 2
Ectopic neurons in Twist1;Wnt1-Cre CKO APCs express sympathetic neuron markers.
In situ hybridization shows that, in Control E11.5 embryos, Hand1 (A) and Hand2 (C) expression is detectable throughout the cNCC-derived APCs, where as expression of other components of the sympathetic neurogenesis pathway, including Phox2b (E), Ascl1 (G), Gata3 (I), TH (K, as assessed via immunohistochemistry), and DBH (M) are not detectable in this tissue. E11.5 Twist1fx/−;Wnt1-Cre(+) mutants, aggregates (arrowheads) express the transcription factors Hand1 (B), Hand2 (D), Phox2b (F), Ascl1 (H), and Gata3 (J), and the norepinephrine biosynthetic enzymes TH (L), and DBH (N). (n = 3).