Histone Deacetylase 2 (HDAC2) Regulates Chromosome Segregation and Kinetochore Function via H4K16 Deacetylation during Oocyte Maturation in Mouse
Figure 2
Increased histone acetylation, and decreased global transcription and histone H3K4 methylation in Hdac1−/+/Hdac2−/− growing oocytes.
(A) Relative abundance of Hdac1 and Hdac2 transcripts in oocytes obtained from WT and Hdac1−/+/Hdac2−/− mice 12 days-of-age. Data are expressed relative to that in WT oocytes. The experiment was performed four times and the data expressed as mean ± SEM. *, P<0.05. (B) Immunocytochemical detection of HDAC1 and HDAC2 in WT and Hdac1−/+/Hdac2−/− oocytes obtained from mice 12-days-of-age. Oocytes were stained with an anti-HDAC1 antibody (Red), anti-HDAC2 antibody (Green) and DAPI (to detect DNA, Blue). Transmitted light micrographs are also shown (DIC). The bar corresponds to 20 µm. (C) Different acetylated histones were analyzed by immunocytochemistry using oocytes obtained from WT and Hdac1−/+/Hdac2−/− mice 12 days-of-age. For each histone variant, at least 20 oocytes for each genotype were analyzed, and the experiment was conducted 3 times. Shown are representative images and only the nucleus is shown. The bar corresponds to 10 µm. (D) Immunocytochemical detection of H3K4 methylation, PolII-CTD-2P, EU and TRP53K379 acethylation in WT and Hdac1−/+/Hdac2−/− oocytes obtained from mice 12-days-of-age. Shown are representative images and only the nucleus is shown. The bar corresponds to 10 µm. (E) Quantification of the data shown in panel D. Nuclear staining intensity of different proteins in WT oocytes was set to 1 and the data are expressed as mean ± SEM. At least 20 oocytes for each genotype and for each detected protein were analyzed; the experiment was conducted three times. *, p<0.05.