Quantitative Analysis of Histone Modifications: Formaldehyde Is a Source of Pathological N6-Formyllysine That Is Refractory to Histone Deacetylases
Figure 3
Formaldehyde is a source of N6-formyllysine.
Formation of N6-formyllysine in (A) in vitro reactions of 1 mM L-lysine with formaldehyde for 2 h at 37°C, and in (B) TK6 cells exposed to formaldehyde, as described in Materials and Methods. Data represent mean ± SD for N = 3, with asterisks denoting statistically significant differences by Student's t-test (p<0.05).