Directed Evolution of a Model Primordial Enzyme Provides Insights into the Development of the Genetic Code
Figure 5
Local environment of hot-spot residues.
(A) Probability that any atom of a residue comes within 0.6 nm of the Cα atom of residue 30 or 61 of subunit A or B during the 10 ns MD simulation of each CM variant as labeled. The probability for residues that lie within ±3 residues of residue 30 or 61 are shown in grey, that for residues further away but in the same subunit are in black, and that for residues in the alternate subunit are in red. (B) and (C) depict the local environment of residues 30 and 61 (black), respectively, in the energy-minimized structure of 9-CM.