Insulin and IGF1 Receptors Are Essential for XX and XY Gonadal Differentiation and Adrenal Development in Mice
Figure 5
Absence of Insr and Igf1r significantly reduces proliferation of somatic cell progenitors prior to sex determination.
Photomicrographs of control and dko embryos at E10.5 (A, scale bar 2.5 mm), E11.5 (B, scale bar 4 mm) and E12.5 (C, scale bar 5 mm). (D) Body weights of control and dko animals at E10.5 (n≥9), E11.5 (n≥22) and E12.5 (n≥11). (E) Scanning electron microscopy pictures of control and dko genital ridges at E11.5 (scale bar 200 µm). A pink overlay marks the genital ridges. (F) FACS analysis was used to determine the number of SF1+ somatic progenitor cells in genital ridges of control and dko animals at E10.5 (n≥9), E11.5 (n≥12), E12.5 (n≥13) and E13.5 (n≥6). (G) Double IF using the gonadal progenitor marker GATA4 (red) along with proliferating marker Ki67 (green) in control and dko genital ridges at E11.5. Quantification of GATA4+/Ki67+ cells in XX and XY genital ridges of control and dko animals at E10.5 (H) and E11.5 (I) revealed a significant reduction in the proliferation rates of somatic gonadal precursors at E10.5 and E11.5 in dko genital ridges. Values are expressed as means ± SEM, *p<0.05, **p<0.01, ***p<0.001 vs control. Scale bar: 100 µm.