Transcription Elongation and Tissue-Specific Somatic CAG Instability
Figure 5
HD transgene expression in R6/1 and R6/2 striatum and cerebellum.
A. Expression of the HD transgene in the striatum and in the cerebellum of R6/1 and R6/2 mice of various ages, as assessed by qRT-PCR using primers located upstream of CAG repeats in HD exon-1. Expression values were normalized to Gapdh expression. Error bars, sem; *, p<0.05; **, p<0.01. B. Correlative analysis of CAG instability rates and HD transgene mRNA levels at 6 weeks of age. C. Absolute quantification of HD transgene transcript levels, as assessed by qRT-PCR using primers upstream of the repeats (upstream primers) and primers amplifying the repeats (CAG primers), from the striatum and cerebellum of R6/1 and R6/2 mice of 6 weeks of age. Corresponding genomic DNAs were used as standards. Levels of PCR products are expressed as equivalent DNA (1 ng eq. DNA corresponds to the PCR signal obtained using 1 ng of DNA). Error bars, sem; *, p<0.05; **, p<0.01.