Global Mapping of Cell Type–Specific Open Chromatin by FAIRE-seq Reveals the Regulatory Role of the NFI Family in Adipocyte Differentiation
Figure 6
NFIA and NFIB are novel regulators of adipocyte differentiation.
(A) Transcriptional regulation of NFI transcription factors during adipocyte differentiation (3T3-F442A). (B) Tissue distribution of the NFI family genes. Expression levels relative to 36B4 in various tissues were determined by qPCR. (C, D) Effects of siRNA-mediated knockdown of NFIA and NFIB on adipogenic gene expression (C) and lipid accumulation in 3T3-L1 adipocytes judged by oil red O staining (D). Knockdown of either NFIA or NFIB resulted in suppression of the induction of PPARγ, C/EBPα and the PPARγ target gene, aP2, as well as increase in lipid accumulation during adipocyte differentiation.