Tsx Produces a Long Noncoding RNA and Has General Functions in the Germline, Stem Cells, and Brain
Figure 5
Tsx KO ES cells exhibit differentiation defects and cell death.
(A) Quantitative RT-PCR analysis of Tsx RNA in male (J1) and female (EL16.7) mouse ES cells during differentiation. Mouse embryonic fibroblasts (MEFs). (B) Phase contrast images (all taken at 4×) of male and female Tsx-null undifferentiated ES cells (d0) and EBs at differentiation d4 and d6. ES cells were differentiated by suspension culture using medium lacking LIF in three independent experiments yielding similar results (images from one experiment are shown). (C) Cell growth of undifferentiated and differentiating male and female null ES cell lines. Equal numbers of cells were plated in triplicate on equal numbers of MEFs, then harvested daily and counted using an automated cell counter.