PDP-1 Links the TGF-β and IIS Pathways to Regulate Longevity, Development, and Metabolism
Figure 5
PDP-1 modulates the expression of insulin genes that possibly feed into the IIS pathway.
Data are representative of one experiment. Error bars represent standard error of the mean within triplicates. All experiments were performed at least twice. (A) The expression of several insulins is elevated in both pdp-1(tm3734) and daf-3(mgDf90) mutants. * p<0.05, ** p<0.007, # a significant reduction in ins-30 levels is observed in pdp-1(tm3734) worms this set but not in others, p<0.03. (B) The same insulins show decreased expression on daf-14(m77) mutants. *p<0.008, **p<0.005, ***p<0.0001. (C) In contrast to the mutants, daf-3::gfp and pdp-1::gfp worms show reduced levels of the insulins tested. *p<0.05, **p<0.005. (D) The trend in Insulin levels are similar between pdp-1(tm3734); daf-2(e1370) and daf-16(mgDf50); daf-2(e1370) double mutants compared to the daf-2(e1370) parental strain. *p<0.03, **p<0.01, # a significant reduction in ins-18 levels is observed in daf-16(mgDf50; daf-2(e1370) mutants this set but not in others, p<0.001. (E) ins-7 levels are drastically elevated in daf-16(mgDf50); daf-2(e1370) and pdp-1(tm3734); daf-2(e1370) worms as compared daf-2(e1370) worms. *p<0.01, **p<0.004.