RACK-1 Acts with Rac GTPase Signaling and UNC-115/abLIM in Caenorhabditis elegans Axon Pathfinding and Cell Migration
Figure 8
Activated MYR::UNC-115 in VD/DD motor neurons is not suppressed by rack-1(tm2262).
A) Quantitation of VD/DD defects caused by expression of MYR::UNC-115 from the unc-115 promoter, which is expressed in the VD/DD neurons. The asterisks represent a test of additivity of the represented genotypes. The double mutant is significantly different from each single, and the double mutant had defects that were significantly stronger than the additive effects of each single. The following formula was used to predict the additive effect of the double mutant based on the single mutant phenotypes: rack-1(tm2262) + lqIs62− (rack-1(tm2262) * lqIs62); or 0.42+0.21− (0.42 * 0.21) = 0.54. At least 100 animals were scored, and p-value significance was determined by Fisher Exact Analysis. B) A VD neuron cell body was laterally displaced (arrow) in a rack-1(tm2262M+); lqIs62[myr::unc-115] animal. rack-1(tm2262) partially suppressed this phenotype compared to lqIs62 alone. Arrowheads indicate VD/DD cell bodies in their normal positions in the ventral nerve cord.