A Yeast Two-Hybrid Screen for SYP-3 Interactors Identifies SYP-4, a Component Required for Synaptonemal Complex Assembly and Chiasma Formation in Caenorhabditis elegans Meiosis
Figure 5
SYP-4 is required for the stabilization of homologous pairing interactions.
(A) Diagram of a C. elegans germline indicating the position of the zones scored in the time-course analysis of homologous pairing. The color codes indicate the genotypes examined in (C). (B) High magnification images of pachytene nuclei from wild type and syp-4 mutants stained with DAPI and hybridized with FISH probes recognizing either the PC end or the non-PC end of chromosome I (green and red, respectively). FISH signals are frequently unpaired in syp-4 mutants. Bars, 2 µm. (C) Graphs depicting the percentage of nuclei carrying paired homologous chromosomes (y-axis) within each zone along the germline (x-axis). The regions being recognized by each of the FISH probes are indicated above the graphs.