Functional Enhancers at the Gene-Poor 8q24 Cancer-Linked Locus
Figure 2
Epigenetic map of 5-Mb around the 8q24 risk loci.
(A) Color coding for 6 combinatorial patterns of epigenomic marks and factors (the means of the tracks in each cluster are shown and demarcated into domains I-VI). (B) Epigenomic profiles derived from LNCaP and PC3 cells, as well as histone acetylation data form these two as well as MCF7 and HCT116 cells. Yellow bars indicate enriched marks, and blue bars indicate negative enrichment. RNA data from Figure 1 (in red) is provided for reference. The top panel presents the results of a spatial clustering analysis, showing a color-coded partitioning of the 5 Mb region into the combinatorial patterns of epigenomic marks and factors as defined in (A). Note a genomic block that includes the MYC gene (domain I, red) and the cancer-linked region is flanked by two H3K27me3-dominated clusters (domain IV, blue). Note also that the risk regions are associated with specific clusters (domain VI, green) that are defined by presence of active chromatin marks in LNCaP and weak or no transcriptional activity.